Crystallization and preliminary X-ray analysis of a chitinase from the fungal pathogen Coccidioides immitis.

نویسندگان

  • T Hollis
  • A F Monzingo
  • K Bortone
  • E Schelp
  • R Cox
  • J D Robertus
چکیده

Chitinase is necessary for fungal growth and cell division and, therefore, is an ideal target for the design of inhibitors which may act as antifungal agents. A chitinase from the fungal pathogen Coccidioides immitis has been expressed as a fusion protein with gluathione-S-transferase (GST), which aids in purification. After cleavage from GST, chitinase was crystallized from 30% PEG 4000 in 0. 1 M sodium acetate pH 4.6. The crystals have a tetragonal crystal lattice and belong to space group P41212 or P43212 and diffract to 2. 2 A resolution. The unit-cell parameters are a = b = 91.2, c = 95.4 A; there is only one chitinase molecule in the asymmetric unit.

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عنوان ژورنال:
  • Acta crystallographica. Section D, Biological crystallography

دوره 54 Pt 6 Pt 2  شماره 

صفحات  -

تاریخ انتشار 1998